The role of cytophilic IgG3 antibody in T cell-mediated resistance to infection with the extracellular bacterium, Pseudomonas aeruginosa. J Immunol 1991 Jan 01;146(1):316-20
Date
01/01/1991Pubmed ID
1898604Scopus ID
2-s2.0-0026034303 (requires institutional sign-in at Scopus site) 25 CitationsAbstract
Previous studies have demonstrated that T lymphocytes from mice immunized with a high m.w. polysaccharide Ag from Fisher-Devlin immunotype I Pseudomonas aeruginosa can adoptively transfer protection against challenge with the homologous bacterial strain to susceptible mice. This T cell-mediated resistance has been found to be B cell dependent, although serum from immunized mice is incapable of passively transferring protection to nonimmune mice. The current studies demonstrate that T cells from immunized mice possess receptors that permit them to be adsorbed to IgG3-secreting hybridomas, but not to IgM-secreting hybridomas. Cross-linking of antibody on the surface of immune T cells results in release of a soluble factor that inhibits bacterial growth. Treatment of T cells to remove cytophilic antibody eliminates their ability to adoptively transfer protection to nonimmune mice, and the protective ability can be restored by co-incubating the T cells with monoclonal P. aeruginosa-specific IgG3 antibody before adoptive transfer to nonimmune mice. These observations are consistent with a model in which T lymphocytes from immunized mice are activated by cross-linking of FcR for IgG3 to secrete an antibacterial lymphokine. The ability of IgG3 at low antibody concentrations to act synergistically with T lymphocytes to inhibit bacterial growth could explain the evolutionary selection of this antibody isotype as the predominant subclass of IgG secreted in response to bacterial capsular polysaccharide Ag.
Author List
Markham RB, Pier GB, Schreiber JRMESH terms used to index this publication - Major topics in bold
AnimalsAntibodies, Bacterial
Antigens, Bacterial
B-Lymphocytes
Female
Hydrogen-Ion Concentration
Immunity, Cellular
Immunization, Passive
Immunoglobulin G
Lymphokines
Mice
Mice, Inbred BALB C
Polysaccharides, Bacterial
Pseudomonas Infections
Pseudomonas aeruginosa
T-Lymphocytes