Organ-specific regulation of pro-inflammatory molecules in heart, lung, and kidney following brain death. J Surg Res 2005 Jan;123(1):118-25
Date
01/18/2005Pubmed ID
15652959DOI
10.1016/j.jss.2004.07.245Scopus ID
2-s2.0-11844268064 (requires institutional sign-in at Scopus site) 80 CitationsAbstract
BACKGROUND: Nonspecific inflammatory events following brain death may increase the intensity of the immunological host response. The present study investigated the course of pro-inflammatory molecules in heart, lung, kidney, and plasma after brain death induction.
MATERIALS AND METHODS: Brain death was induced in five pigs by inflation of an intracranial Foley catheter and five pigs were sham-operated as controls. Each experiment was terminated 6 h after brain death/sham operation and the organs were harvested. We measured the mRNA and protein levels for TNF-alpha, IL-1beta, and IL-6 in heart, lung, kidney, and plasma. Additionally, the mRNA expression for IL-6R, ICAM-1, MCP-1, and TGF-beta was determined in each organ.
RESULTS: After 6 h, the plasma cytokine levels were higher in the brain-dead animals than in the sham-operated. In heart, lung, and kidney there was an increase in IL-6 and IL-1beta following brain death, while TNF-alpha was up-regulated in lung only (P < 0.05). MCP-1 and TGF-beta were significantly higher in heart and lung and IL-6R increased in heart after brain death (P < 0.05).
CONCLUSIONS: Brain death was associated with non-uniform cytokine expression patterns in the investigated organs. These expression patterns may cause variable pro-inflammatory priming resulting in different degrees of damage and explain the organ-specific variation in outcomes after transplantations.
Author List
Skrabal CA, Thompson LO, Potapov EV, Southard RE, Joyce DL, Youker KA, Noon GP, Loebe MMESH terms used to index this publication - Major topics in bold
AnimalsBrain Death
Cytokines
Female
Gene Expression Profiling
Intercellular Adhesion Molecule-1
Interleukin-1
Interleukin-6
Kidney
Lung
Male
Myocardium
Organ Specificity
RNA, Messenger
Receptors, Interleukin-6
Swine
Tumor Necrosis Factor-alpha
