Black Raspberries and Their Anthocyanin and Fiber Fractions Alter the Composition and Diversity of Gut Microbiota in F-344 Rats. Nutr Cancer 2017;69(6):943-951
Date
07/19/2017Pubmed ID
28718724Pubmed Central ID
PMC6139254DOI
10.1080/01635581.2017.1340491Scopus ID
2-s2.0-85024493672 (requires institutional sign-in at Scopus site) 86 CitationsAbstract
Natural compounds can alter the diversity and composition of the gut microbiome, potentially benefiting our health. We previously demonstrated chemopreventive effects of black raspberries (BRBs) in colorectal cancer, which is associated with gut dysbiosis. To investigate the effects of whole BRBs and their fractions on gut microbiota, we fed F-344 rats a control diet, 5% BRBs, the BRB anthocyanin fraction, or the BRB residue fraction for 6 weeks. Feces were collected at baseline and at weeks 3 and 6, and bacterial sequence counts were analyzed. We observed distinct patterns of microbiota from different diet groups. Beta diversity analysis suggested that all diet groups exerted time-dependent changes in the bacterial diversity. Hierarchical clustering analysis revealed that post-diet fecal microbiota was segregated from baseline fecal microbiota within each diet. It is interesting to note that fractions of BRBs induced different changes in gut bacteria compared to whole BRBs. The abundance of specific microbial species known to have anti-inflammatory effects, such as Akkermansia and Desulfovibrio, was increased by whole BRBs and their residue. Further, butyrate-producing bacteria, e.g., Anaerostipes, were increased by whole BRBs. Our results suggest that whole BRBs and their fractions alter the gut microbiota in ways that could significantly influence human health.
Author List
Pan P, Lam V, Salzman N, Huang YW, Yu J, Zhang J, Wang LSAuthor
Nita H. Salzman MD, PhD Center Director, Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsAnthocyanins
Clostridiales
Desulfovibrio
Diet
Dietary Fiber
Feces
Fruit
Gastrointestinal Microbiome
Rats
Rats, Inbred F344
Rubus
Verrucomicrobia
