Nerve growth factor treatment enhances release of immunoreactive calcitonin gene-related peptide but not substance P from spinal dorsal horn slices in rats. Neurosci Lett 2004 Jun 17;363(3):239-42
Date
06/09/2004Pubmed ID
15182951DOI
10.1016/j.neulet.2004.03.068Scopus ID
2-s2.0-2942620591 (requires institutional sign-in at Scopus site) 21 CitationsAbstract
The effect of systemic nerve growth factor (NGF) on neuropeptide content and capsaicin-evoked release of neuropeptide from in vitro spinal cord dorsal horn slices was examined. Rats were injected subcutaneously every other day with murine NGF (mNGF) 1 mg/kg or saline for 7 days, or mNGF 0.1/kg, mNGF 1 mg/kg or saline for 13 days. Lumbar dorsal horn slices of the rat spinal cord from all groups showed a significant increase in immunoreactive calcitonin gene-related peptide (CGRP) release upon exposure to capsaicin. This release was enhanced in rats pretreated with mNGF 1 mg/kg for 7 days, but not after 13 days. No enhancement was seen after 7 or 13 days in any treatment group for immunoreactive substance P release. Upon examination of neuropeptide content in dorsal horn, no significant differences were noted between treatment groups. The increased iCGRP release from dorsal horn slices suggests a preferential release of CGRP and provides further evidence that NGF indirectly plays a role in the modulation of inflammation through the regulation of neuropeptide release.
Author List
Bowles WR, Sabino M, Harding-Rose C, Hargreaves KMMESH terms used to index this publication - Major topics in bold
AnimalsCalcitonin Gene-Related Peptide
Capsaicin
Dose-Response Relationship, Drug
Drug Administration Schedule
Drug Interactions
In Vitro Techniques
Male
Mice
Nerve Growth Factors
Posterior Horn Cells
Radioimmunoassay
Rats
Rats, Sprague-Dawley
Spinal Cord
Substance P
