Cell Surface Proteomics of N-Linked Glycoproteins for Typing of Human Lymphocytes. Proteomics 2017 Oct;17(19)
Date
08/24/2017Pubmed ID
28834292Pubmed Central ID
PMC5633517DOI
10.1002/pmic.201700156Scopus ID
2-s2.0-85030722078 (requires institutional sign-in at Scopus site) 16 CitationsAbstract
Lymphocytes are immune cells that are critical for the maintenance of adaptive immunity. Differentiation of lymphoid progenitors yields B-, T-, and NK-cell subtypes that individually correlate with specific forms of leukemia or lymphoma. Therefore, it is imperative a precise method of cell categorization is utilized to detect differences in distinct disease states present in patients. One viable means of classification involves evaluation of the cell surface proteome of lymphoid malignancies. Specifically, this manuscript details the use of an antibody independent approach known as Cell Surface Capture Technology, to assess the N-glycoproteome of four human lymphocyte cell lines. Altogether, 404 cell surface N-glycoproteins were identified as markers for specific cell types involved in lymphocytic malignancies, including 82 N-glycoproteins that had not been previously been described for B or T cells within the Cell Surface Protein Atlas. Comparative analysis, hierarchical clustering techniques, and label-free quantitation were used to reveal proteins most informative for each cell type. Undoubtedly, the characterization of the cell surface proteome of lymphoid malignancies is a first step toward improving personalized diagnosis and treatment of leukemia and lymphoma.
Author List
Haverland NA, Waas M, Ntai I, Keppel T, Gundry RL, Kelleher NLMESH terms used to index this publication - Major topics in bold
Biomarkers, TumorCell Membrane
Cells, Cultured
Glycoproteins
Humans
Leukemia
Lymphocytes
Lymphoma
Proteome
Proteomics
