Faculty Collaboration Database

Quantitation of corneal inflammation by chemiluminescense. Arch Ophthalmol 1986 Oct;104(10):1540-4

Date

10/01/1986

Pubmed ID

3094481

DOI

10.1001/archopht.1986.01050220134041

Scopus ID

2-s2.0-0022543160 (requires institutional sign-in at Scopus site)   9 Citations

Abstract

Various inflammatory agents, including Pseudomonas aeruginosa, bacterial filtrates, endotoxin, and phorbol myristate acetate were found to induce significant increases in corneal chemiluminescense (CLM). Disruption of polymorphonuclear leukocytes within corneas by sonication, freeze-thawing or cryotherapy, or reduction of corneal infiltration by induction of neutropenia resulted in marked decreases of CLM. Increased corneal CLM was associated with significant increases in corneal thickness and water content. Oxygen-free radical scavengers significantly inhibited CLM of experimentally infected corneas in vitro, as did the anti-inflammatory agents prednisolone acetate, indomethacin, and salicylic acid. In vivo therapy of infected corneas with prednisolone resulted in significant reductions in corneal CLM, thickness, and water content compared with saline-treated eyes. The CLM assay is a simple technique that allows quantitation of corneal inflammation and evaluation of the effect of therapeutic agents on corneal inflammation.

Author List

Chusid MJ, Shea ML

MESH terms used to index this publication - Major topics in bold

Animals
Cornea
Cryosurgery
Endotoxins
Escherichia coli
Guinea Pigs
Keratitis
Luminescent Measurements
Luminol
Mannitol
Neutrophils
Prednisolone
Pseudomonas Infections
Pseudomonas aeruginosa
Salicylates
Sodium Hydroxide
Tetradecanoylphorbol Acetate