Oxygen free radicals abolish endothelium-dependent relaxation in diabetic rat aorta. Am J Physiol 1988 Oct;255(4 Pt 2):H825-33
Date
10/01/1988Pubmed ID
3140677DOI
10.1152/ajpheart.1988.255.4.H825Scopus ID
2-s2.0-0023713429 (requires institutional sign-in at Scopus site) 208 CitationsAbstract
The present experiments were conducted to determine whether endothelium-dependent relaxation is impaired in chronic (10-12 wk), streptozotocin-induced diabetic rat aortas and to determine the specificity and sensitivity of diabetic vasculature to oxygen-derived free radicals. Endothelium-dependent relaxation by acetylcholine and ADP was severely impaired in diabetic rat aorta, whereas endothelium-independent relaxation by nitroglycerin or papaverine was not impaired. Exposure to a free radical-generating system of xanthine plus xanthine oxidase caused a marked and prolonged relaxation in diabetic but not control vessels. Relaxation could not be prevented by the cyclooxygenase inhibitor indomethacin or the lipoxygenase inhibitor nordihydroguaiaretic acid but was attenuated or blocked by catalase. After free radical exposure, aortic rings were washed, reequilibrated, and contracted with a submaximal concentration of norepinephrine. In free radical-exposed vessels, endothelium-dependent relaxation by acetylcholine was reduced by 50% in nondiabetic vessels and abolished in diabetic vessels. Nevertheless, diabetic vessels could still be fully relaxed by nitroglycerin or papaverine. These results suggest selective impairment of endothelium-dependent relaxation in chronic diabetic rat aortas with particular sensitivity to free radical-induced damage.
Author List
Pieper GM, Gross GJMESH terms used to index this publication - Major topics in bold
AcetylcholineAdenosine Diphosphate
Animals
Aorta, Thoracic
Ascorbic Acid
Catalase
Diabetes Mellitus, Experimental
Endothelium, Vascular
Free Radicals
In Vitro Techniques
Indomethacin
Male
Masoprocol
Nitroglycerin
Norepinephrine
Papaverine
Rats
Rats, Inbred Strains
Reference Values
Vasodilation
