Explant culture of rat colon: a model system for studying metabolism of chemical carcinogens. In Vitro 1978 Oct;14(10):868-77
Date
10/01/1978Pubmed ID
102584DOI
10.1007/BF02616157Scopus ID
2-s2.0-0018076177 (requires institutional sign-in at Scopus site) 52 CitationsAbstract
An explant culture system has been developed for the long-term maintenance of colonic tissue from the rat. Explants of 1 cm2 in size were placed in tissue-culture dishes to which was added 2 ml of CMRL-1066 medium supplemented with glucose, hydrocortisone, beta-retinyl acetate, and either 2.5% bovine albumin or 5% fetal bovine serum. The dishes were placed in a controlled-atmosphere chamber which was gassed with 95% O2 and 5% CO2. The chamber then was placed on a rocker platform which rocked at 10 cycles per min causing the medium to flow intermittently over the epithelial surface. The explants were incubated at 30 degrees C. The viability of the tissue was measured both by incorporation of specific precursors into cellular macromolecules and by monitoring of tissue morphology with light and electron microscopy. Cultured rat colon was able to metabolize benzo[alpha]pyrene, 7,12-dimethylbenz[alpha]anthracene, aflatoxin B1, dimethylnitrosamine, 1,2-dimethylhydrazine, and methylazoxymethanol acetate into chemical species that bind to cellular DNA and protein.
Author List
Autrup H, Stoner GD, Jackson F, Harris CC, Shamsuddin AK, Barrett LA, Trump BFMESH terms used to index this publication - Major topics in bold
9,10-Dimethyl-1,2-benzanthraceneAflatoxins
Animals
Benzopyrenes
Carcinogens
Colon
Dimethylhydrazines
Dimethylnitrosamine
Intestinal Mucosa
Male
Methylazoxymethanol Acetate
Models, Biological
Organ Culture Techniques
Rats
