Faculty Collaboration Database

Isolation and characterization of epithelial cells from bovine pancreatic duct. In Vitro 1978 Jul;14(7):581-90

Date

07/01/1978

Pubmed ID

566723

DOI

10.1007/BF02617917

Scopus ID

2-s2.0-0017859568 (requires institutional sign-in at Scopus site)   44 Citations

Abstract

Epithelial cells derived from bovine pancreatic duct have been grown continuously in culture for 30 weeks (approximately 90 doublings of the cell population). The cells were grown in Eagle's minimal essential medium supplemented with 10% heat-inactivated fetal bovine serum, 2 mM glutamine, 0.1 mM nonessential amino acids, and antibiotics. In confluent cultures, the cells are multilayered and form circular structures. When tested at various passages, the cells neither formed colonies in soft agar nor produced tumors after inoculation into athymic, nude mice. Hydrocortisone (1 and 5 microgram per ml) and insulin (1,5 and 10 microgram per ml) had no effect on the growth of the cells. beta-Retinyl acetate inhibited growth rate and cell yield at a concentration of 5 microgram per ml but was not growth-inhibitory at lower concentrations. By electron microscopy the cells have numerous mitochondria, Golgi and microvilli. Mucous droplets were observed in a small proportion of the cells. Desmosome-like structures and occluding junctions were observed more frequently between cells that had been transferred as aggregates than between cells transferred as single cells. Cytochemical studies indicated that some cells produce PAS positive granules that were not removed after treatment of the cultures with diastase. Eleven cell clones were isolated from the mass culture. The growth rates of the clones are different as well as the period of time in which the clones can be propagated in vitro.

Author List

Stoner GD, Harris CC, Bostwick DG, Jones RT, Trump BF, Kingsbury EW, Fineman E, Newkirk C

MESH terms used to index this publication - Major topics in bold

Animals
Cattle
Cell Division
Cell Line
Cell Separation
Chromosomes
Clone Cells
Culture Media
Epithelial Cells
Hydrocortisone
Insulin
Karyotyping
Male
Pancreatic Ducts
Retinaldehyde