Explant culture of human peripheral lung. I. Metabolism of benzo[alpha]pyrene. Lab Invest 1978 Jun;38(6):685-92
Date
06/01/1978Pubmed ID
661225Scopus ID
2-s2.0-0018095149 (requires institutional sign-in at Scopus site) 56 CitationsAbstract
Human lung explants have been maintained in vitro for a period of 25 days. Autoradiographic studies indicated that the broncholar epithelial cells, type 2 alveolar epithelial cells, and stromal fibroblasts incorporated 3H-thymidine during the culture. After 7 to 10 days, type 2 cells were the predominant alveolar epithelial cell type. Lamellar inclusion bodies were released from the type 2 cells and accumulated in the alveolar spaces. The metabolism of benzo[alpha]pyrene (BP) in human lung explants cultured for up to 7 days was investigated. Human lung explants had measurable aryl hydrocarbon hydroxylase activity and could metabolize BP into forms that were bound to cellular DNA and protein. Peripheral lung had significantly lower aryl hydrocarbon hydroxylase activity than cultured bronchus but both tissues had similar binding levels of BP to DNA. Radioautographic studies indicated that all cell types in the peripheral lung can metabolize BP. The major ethylacetate extractable metabolites of BP formed by peripheral lung were tetrols and trans-7,8-diol. The primary water-soluble metabolite released with arylsulfatase and beta-glucuronidase was 3-hydroxybenzo[alpha]pyrene.
Author List
Stoner GD, Harris CC, Autrup H, Trump BF, Kingsbury EW, Myers GAMESH terms used to index this publication - Major topics in bold
Aryl Hydrocarbon HydroxylasesBenzopyrenes
Culture Techniques
DNA
Epithelium
Fibroblasts
Humans
Lung
Microscopy, Electron
Mitosis
Protein Binding
Time Factors
