Faculty Collaboration Database

Negative regulation of monocyte adhesion to arterial elastic laminae by signal regulatory protein alpha and Src homology 2 domain-containing protein-tyrosine phosphatase-1. J Biol Chem 2005 Nov 25;280(47):39294-301

Date

09/15/2005

Pubmed ID

16159885

DOI

10.1074/jbc.M503866200

Scopus ID

2-s2.0-28244493621 (requires institutional sign-in at Scopus site)   39 Citations

Abstract

Elastic laminae are extracellular matrix constituents that not only contribute to the stability and elasticity of arteries but also play a role in regulating arterial morphogenesis and pathogenesis. We demonstrate here that an important function of arterial elastic laminae is to prevent monocyte adhesion, which is mediated by the inhibitory receptor signal regulatory protein (SIRP) alpha and Src homology 2 domain-containing protein-tyrosine phosphatase (SHP)-1. In a matrix-based arterial reconstruction model in vivo, elastic laminae were resistant to leukocyte adhesion and transmigration compared with the collagen-dominant arterial adventitia. The density of leukocytes within the elastic lamina-dominant media was about 58-70-fold lower than that within the adventitia from 1 to 30 days. An in vitro assay confirmed the inhibitory effect of elastic laminae on monocyte adhesion. The exposure of monocytes to elastic laminae induced activation of SIRP alpha, which in turn activated SHP-1. Elastic lamina degradation peptides extracted from arterial specimens could also activate SIRP alpha and SHP-1. The knockdown of SIRP alpha and SHP-1 by specific small interfering RNA diminished the inhibitory effect of elastic laminae, resulting in a significant increase in monocyte adhesion. These observations suggest that SIRP alpha and SHP-1 potentially mediate the inhibitory effect of elastic laminae on monocyte adhesion.

Author List

Liu SQ, Alkema PK, Tieché C, Tefft BJ, Liu DZ, Li YC, Sumpio BE, Caprini JA, Paniagua M

Author

Brandon J. Tefft PhD Associate Professor in the Biomedical Engineering department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Arteries
Cell Adhesion
Elastic Tissue
In Vitro Techniques
Intracellular Signaling Peptides and Proteins
Leukocytes
Monocytes
Phosphorylation
Protein Phosphatase 1
Protein Tyrosine Phosphatase, Non-Receptor Type 6
Protein Tyrosine Phosphatases
RNA, Small Interfering
Rats
Rats, Sprague-Dawley
Receptors, Cell Surface