Factors Known to Influence the Development of Necrotizing Enterocolitis to Modify Expression and Activity of Intestinal Alkaline Phosphatase in a Newborn Neonatal Rat Model. Eur J Pediatr Surg 2019 Jun;29(3):290-297
Date
05/04/2018Pubmed ID
29723880DOI
10.1055/s-0038-1646959Scopus ID
2-s2.0-85047363575 (requires institutional sign-in at Scopus site) 8 CitationsAbstract
INTRODUCTION: Prematurity, formula feeding, and early weaning strongly influence enterocyte differentiation. Intestinal alkaline phosphatase (IAP), an endogenous protein expressed in the intestines, is one enzyme that is affected by these factors. IAP supplementation decreases the severity of necrotizing enterocolitis (NEC) injury. We, therefore, hypothesized that prematurity predisposes this population to NEC due to IAP deficiency and investigated IAP expression and function in a neonatal rat model.
MATERIALS AND METHODS: Pre- and full-term newborn Sprague-Dawley rat pups were sacrificed on consecutive days of life both after vaginal or cesarean birth and following either breast or formula feeding.
RESULTS: Compared with controls, cesarean delivery and formula feeding are associated with lower levels of IAP. The formula-fed pups continued to have low baseline IAP activity. Neither prematurity nor formula feeding led to differences of intestinal injury.
CONCLUSION: Prematurity and formula feeding are associated with inhibition of IAP expression and activity. Both may increase the risk of NEC and early enteral supplementation of IAP to newborns at risk of NEC may be of therapeutic benefit.
Author List
Rentea RM, Rentea MJ, Biesterveld B, Liedel JL, Gourlay DMAuthor
David M. Gourlay MD Chief, Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Alkaline PhosphataseAnimals
Animals, Newborn
Biomarkers
Cesarean Section
Enterocolitis, Necrotizing
Humans
Infant Formula
Infant, Newborn
Infant, Premature, Diseases
Isoenzymes
Rats
Rats, Sprague-Dawley
Risk Factors
