Enzymatic amplification of platelet-specific messenger RNA using the polymerase chain reaction. J Clin Invest 1988 Aug;82(2):739-43
Date
08/01/1988Pubmed ID
3403726Pubmed Central ID
PMC303572DOI
10.1172/JCI113656Scopus ID
2-s2.0-0023762799 (requires institutional sign-in at Scopus site) 194 CitationsAbstract
Human platelets are derived from megakaryocytes as anucleate cells, and thus contain only vestigial amounts of RNA capable of being transcribed into protein. This has greatly hampered efforts to study directly platelet-specific gene products and their associated polymorphisms. In this report, we describe direct amplification, using the polymerase chain reaction, of platelet-derived mRNA in amounts sufficient to permit detailed analysis, such as restriction mapping and nucleotide sequencing. The ability to generate large amounts of cDNA from platelet-specific mRNA sequences should make possible direct molecular characterization of normal platelet proteins, and facilitate the investigation of a wide variety of inherited platelet disorders.
Author List
Newman PJ, Gorski J, White GC 2nd, Gidwitz S, Cretney CJ, Aster RHAuthor
Gilbert C. White MD Professor in the Medicine department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
Blood PlateletsCloning, Molecular
DNA
DNA-Directed DNA Polymerase
Gene Amplification
Humans
Nucleic Acid Hybridization
Platelet Membrane Glycoproteins
RNA, Messenger
