Flavobacterium johnsoniae SprA is a cell surface protein involved in gliding motility. J Bacteriol 2007 Oct;189(19):7145-50
Date
07/24/2007Pubmed ID
17644580Pubmed Central ID
PMC2045224DOI
10.1128/JB.00892-07Scopus ID
2-s2.0-34948891544 (requires institutional sign-in at Scopus site) 42 CitationsAbstract
Flavobacterium johnsoniae cells glide rapidly over surfaces by an unknown mechanism. Transposon-induced sprA mutants formed nonspreading colonies on agar, and the cells examined in wet mounts were deficient in attachment to surfaces and were almost completely nonmotile. Exposure of intact cells to proteinase K cleaved the 270-kDa SprA into several large peptides, suggesting that it is partially exposed on the cell surface.
Author List
Nelson SS, Glocka PP, Agarwal S, Grimm DP, McBride MJMESH terms used to index this publication - Major topics in bold
Bacterial ProteinsBacteriophages
Chitin
Cloning, Molecular
DNA Transposable Elements
Endopeptidase K
Flavobacterium
Gene Expression Regulation, Bacterial
Genetic Complementation Test
Membrane Proteins
Molecular Sequence Data
Mutagenesis, Insertional
Mutation
Sequence Analysis, DNA
