Medical College of Wisconsin
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Adding ROS quenchers to cold K+ cardioplegia reduces superoxide emission during 2-hour global cold cardiac ischemia. J Cardiovasc Pharmacol Ther 2012 Mar;17(1):93-101

Date

02/02/2011

Pubmed ID

21282477

Pubmed Central ID

PMC3226871

DOI

10.1177/1074248410389815

Scopus ID

2-s2.0-84856953884 (requires institutional sign-in at Scopus site)   8 Citations

Abstract

We reported that the combination of reactive oxygen species (ROS) quenchers Mn(III) tetrakis (4-benzoic acid) porphyrin (MnTBAP), catalase, and glutathione (MCG) given before 2 hours cold ischemia better protected cardiac mitochondria against cold ischemia and warm reperfusion (IR)-induced damage than MnTBAP alone. Here, we hypothesize that high K(+) cardioplegia (CP) plus MCG would provide added protection of mitochondrial bioenergetics and cardiac function against IR injury. Using fluorescence spectrophotometry, we monitored redox balance, ie reduced nicotinamide adenine dinucleotide and flavin adenine dinucleotide (NADH/FAD), superoxide (O(2) (•-)), and mitochondrial Ca(2+) (m[Ca(2+)]) in the left ventricular free wall. Guinea pig isolated hearts were perfused with either Krebs Ringer's (KR) solution, CP, or CP + MCG, before and during 27°C perfusion followed immediately by 2 hours of global ischemia at 27°C. Drugs were washed out with KR at the onset of 2 hours 37°C reperfusion. After 120 minutes warm reperfusion, myocardial infarction was lowest in the CP + MCG group and highest in the KR group. Developed left ventricular pressure recovery was similar in CP and CP + MCG and was better than in the KR group. O(2) (•-), m[Ca(2+)], and NADH/FAD were significantly different between the treatment and KR groups. O(2) (•-) was lower in CP + MCG than in the CP group. This study suggests that CP and ROS quenchers act in parallel to improve mitochondrial function and to provide protection against IR injury at 27°C.

Author List

Aldakkak M, Stowe DF, Heisner JS, Riess ML, Camara AK

Author

Amadou K. Camara PhD Professor in the Anesthesiology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Animals
Cardioplegic Solutions
Cold Ischemia
Guinea Pigs
Heart Arrest, Induced
Heart Rate
Hypertonic Solutions
Myocardial Ischemia
Myocardial Reperfusion Injury
Potassium Compounds
Random Allocation
Reactive Oxygen Species
Superoxides
Time Factors