Faculty Collaboration Database

Expression of GD3 ganglioside in childhood T-cell lymphoblastic malignancies. Cancer Res 1987 Mar 15;47(6):1724-30

Date

03/15/1987

Pubmed ID

3493067

Scopus ID

2-s2.0-0023159987 (requires institutional sign-in at Scopus site)   49 Citations

Abstract

Lymphoblasts from seven children with T-cell lymphoblastic malignancies and three children with non-T, non-B acute lymphoblastic leukemia (ALL) were analyzed for ganglioside content. Nonmalignant T-cells from thymus served as controls. Both ganglioside and glycoprotein sialic acid were increased approximately 3-3.5-fold in T-cell disease compared to thymic tissue when expressed on a per cell basis, but not on a per milligram protein basis. Thin-layer chromatography of the isolated ganglioside fraction from T-cell lymphoblasts revealed two major resorcinol-positive bands. One ganglioside comigrated with II3-alpha-N-acetylneuraminosyllactosylceramide (GM3), the major ganglioside in normal lymphoid tissue, and the other ganglioside comigrated with authentic II3-alpha-N-acetylneuraminosyl-alpha 2----8-N-acetylneuraminosyllactosylceramide (GD3) in three different solvent systems. Neuraminidase treatment of the latter ganglioside yielded GM3 and lactosyl ceramide, hydrolysis products of GD3. Scanning densitometry revealed that whereas thymus cells contained 97% GM3 and 3% GD3, T-cell lymphoblasts contained from 22 to 86% GD3 and a corresponding decrease in GM3. The shift to increased GD3 was observed in the blasts from all seven T-cell patients, but not in the blasts from the non-T, non-B patients studied. Only trace quantities of GD3 were detected from two continuous T-ALL cell lines, HSB2 and RPMI 8402. The results demonstrate a consistently significant increase in ganglioside GD3 in uncultured, patient-derived T-cell ALL lymphoblasts when compared to non-T-cell ALL and normal lymphoid tissue. Therefore, GD3 may represent a tumor-associated antigen for the T-cell subclass of childhood lymphoblastic malignancy.

Author List

Merritt WD, Casper JT, Lauer SJ, Reaman GH

MESH terms used to index this publication - Major topics in bold

Adolescent
Cell Line
Child
Child, Preschool
Chromatography, Thin Layer
Densitometry
Female
G(M3) Ganglioside
Gangliosides
Humans
Leukemia, Lymphoid
Male
N-Acetylneuraminic Acid
Neuraminidase
Sialic Acids
T-Lymphocytes