Recording SOCE Activity in Neurons by Patch-Clamp Electrophysiology and Microfluorometric Calcium Imaging. Methods Mol Biol 2018;1843:41-53
Date
09/12/2018Pubmed ID
30203275DOI
10.1007/978-1-4939-8704-7_3Scopus ID
2-s2.0-85053995005 (requires institutional sign-in at Scopus site) 2 CitationsAbstract
Store-operated Ca2+ entry (SOCE) is a Ca2+ influx pathway at the plasma membrane that replenishes intracellular Ca2+ stores in response to depletion of Ca2+ stores. The SOC current, also known as the Ca2+ release-activated Ca2+ current (ICRAC), has a small conductance, which makes selective recording difficult. This challenge may be addressed using techniques based on identification of Ca2+ influx patch-clamp electrophysiological recording and measurement of cytoplasmic Ca2+ accumulation with Ca2+-sensitive fluorophores. Here, we describe specific methods for studying SOCE using these approaches in rat dorsal root ganglion neurons.
Author List
Wu HE, Gemes G, Hogan QHAuthor
Quinn H. Hogan MD Professor in the Anesthesiology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCalcium
Calcium Channel Blockers
Calcium Channels
Calcium Signaling
Cytophotometry
Electrophysiological Phenomena
Ion Channel Gating
Mice
Molecular Imaging
Neurons
Patch-Clamp Techniques
Rats
Single-Cell Analysis
