Dye-mediated photosensitization of murine neuroblastoma cells. Cancer Res 1986 Apr;46(4 Pt 2):2072-6
Date
04/01/1986Pubmed ID
3512078Scopus ID
2-s2.0-0022624945 (requires institutional sign-in at Scopus site) 71 CitationsAbstract
The purpose of this study was to determine if photosensitization mediated by the fluorescent dye, merocyanine 540, could be used to preferentially kill murine neuroblastoma cells in simulated autologous remission marrow grafts. Simultaneous exposure of Neuro 2a or NB41A3 neuroblastoma cells to merocyanine 540 and white light reduced the concentration of in vitro-clonogenic tumor cells 50,000-fold. By contrast, the same treatment had little effect on the graft's ability to rescue lethally irradiated syngeneic hosts. Lethally irradiated C57BL/6J X A/J F1 mice transplanted with photosensitized mixtures of neuroblastoma cells and normal marrow cells (1:100 or 1:10) survived without developing neuroblastomas. It is conceivable that merocyanine 540-mediated photosensitization will prove useful for the extracorporeal purging of residual neuroblastoma cells from human autologous remission marrow grafts.
Author List
Sieber F, Sieber-Blum MAuthor
Fritz Sieber PhD Professor in the Pediatrics department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsBone Marrow
Bone Marrow Transplantation
Cell Line
Cell Survival
Female
Hematopoietic Stem Cells
Mice
Mice, Inbred C57BL
Neuroblastoma
Photochemotherapy
Pyrimidinones
Radiation-Sensitizing Agents
Transplantation, Autologous
Trypsin