Effect of phagocytosis and ionophores on release and metabolism of arachidonic acid from human neutrophils. Lipids 1981 Feb;16(2):120-4
Date
02/01/1981Pubmed ID
6787359DOI
10.1007/BF02535685Scopus ID
2-s2.0-0019522180 (requires institutional sign-in at Scopus site) 36 CitationsAbstract
Challenge of human neutrophils prelabeled with [3H] arachidonate and [14C] palmitate or [14C]-stearate with opsonized zymosan or the Ca2+ ionophores A23187 or Ionomycin caused the release of [3H], but not [14C], fatty acid. With the ionophores, but not zymosan, considerable conversion of the [3H] arachidonate to hydroxyeicosatetraenoates occurred. Although various isomers were recovered, the 5-hydroxyeicosatetraenoate appeared to be the major product. In these experiments, no [14C] products were detected such as lysophospholipid, diglyceride or monoglyceride. Although no definite statement can be made about the mechanism of release of arachidonate, our data are most easily interpreted as the result of the action of a phospholipase A2.
Author List
Walsh CE, Dechatelet LR, Thomas MJ, O'Flaherty JT, Waite MMESH terms used to index this publication - Major topics in bold
Anti-Bacterial AgentsArachidonic Acids
Calcimycin
Ethers
Glycerides
Humans
Ionomycin
Isomerism
Neutrophils
Palmitic Acids
Phagocytosis
Phospholipids
Stearates
Zymosan