Faculty Collaboration Database

Alterations in biochemical functions during hyperthermic isolation-perfusion of the human liver. J Surg Res 1986 Oct;41(4):432-8

Date

10/01/1986

Pubmed ID

3773503

DOI

10.1016/0022-4804(86)90058-2

Scopus ID

2-s2.0-0023006791 (requires institutional sign-in at Scopus site)   10 Citations

Abstract

Hyperthermia (42-42.5 degrees) was applied to the liver of eight patients with cancer in the liver by a technique of isolation-perfusion. Hepatic functional integrity was assessed during perfusion through measurement of multiple perfusate constituents. Data from seven perfusions were available for analysis. During perfusion there was an increase in perfusate lactate, pyruvate, glucose, urea, potassium, alkaline phosphatase, SGOT, and LDH. All increases in these constituents were significant (P less than 0.05) except for potassium. Lactate accumulated throughout the perfusion from an initial level of 3.8 +/- 1.0 mM to 7.6 +/- 3.5 mM at 4 hr. Pyruvate increased over the first 3 hr of perfusion from 0.14 +/- 0.06 mM to 0.80 +/- 0.37 mM before declining to 0.54 +/- 0.24 mM at 4 hr. The L/P (lactate/pyruvate) ratio decreased during perfusion to less than 10 in the first 2 hr, but rose to within normal limits by the end of perfusion. The decreases in L/P ratios were significant (P less than 0.05). Initially there was a rapid rise in perfusate glucose concentrations from 4.5 +/- 0.8 mM to 20.7 +/- 5.4 mM at 2 hr with nonsignificant changes thereafter. Urea levels increased from 0.64 +/- 0.22 mM to 1.92 +/- 0.76 mM. Perfusate potassium increased from the initial level of 7.0 +/- 1.0 mM during perfusion to 8.3 +/- 1.7 mM at 2 hr before declining. SGOT, LDH, and alkaline phosphatase increased during perfusion from 21 +/- 15, 142 +/- 48, and 16 +/- 6 to 176 +/- 22, 472 +/- 53 and 52 +/- 42, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

Author List

Skibba JL, Quebbeman EJ, Kalbfleisch JH, Sonsalla JC, Petroff RJ Jr

MESH terms used to index this publication - Major topics in bold

Adult
Aged
Alkaline Phosphatase
Aspartate Aminotransferases
Blood Glucose
Blood Urea Nitrogen
Carcinoma
Humans
Hyperthermia, Induced
Ketone Bodies
L-Lactate Dehydrogenase
Lactates
Liver
Liver Neoplasms
Melanoma
Middle Aged
Perfusion
Potassium
Pyruvates