Faculty Collaboration Database

Hexosaminidase: a marker for healing after ischemic gut injury. J Surg Res 1986 Apr;40(4):362-7

Date

04/01/1986

Pubmed ID

3702389

DOI

10.1016/0022-4804(86)90200-3

Scopus ID

2-s2.0-0022608943 (requires institutional sign-in at Scopus site)   8 Citations

Abstract

Serum hexosaminidase activity (HEX) is elevated with ischemic gut injury. To determine if subsequent decreases in HEX correlate with gut healing, 97 weanling rats were subjected to laparotomy at which alternate vascular bundles were ligated along the base of the entire anterior mesenteric artery arcade. Fifteen rats served as preoperative controls. After recovery, rats were allowed ad lib food and water. Groups were then killed at intervals, blood was drawn for HEX determination, and samples of small bowel were taken for histological evaluation. Microscopically, focal ischemic necrosis began at 6 hr. Between 12 and 48 hr, cellular changes were consistent with progressive ischemic injury. Evidence of healing was apparent beginning at 5 days and these histological changes correlated with changes in HEX. Thus HEX proves useful as a marker for gut healing in this model.

Author List

Lobe TE, Dobkin EE, Rassin DK, Gourley WK, Oldham KT, Bhatia J

MESH terms used to index this publication - Major topics in bold

Animals
Clinical Enzyme Tests
Enterocolitis, Pseudomembranous
Hexosaminidases
Intestinal Mucosa
Intestines
Ischemia
Male
Necrosis
Rats
Rats, Inbred Strains
Time Factors
Wound Healing