Vocal cord augmentation with cultured autologous fibroblasts. Bull Exp Biol Med 2000 Aug;130(8):790-2
Date
02/15/2001Pubmed ID
11177246DOI
10.1007/BF02766097Scopus ID
2-s2.0-0035122518 (requires institutional sign-in at Scopus site) 5 CitationsAbstract
Bovine collagen is an acceptable agent for vocal cord medialization; however, it produces only a temporary effect. As a foreign protein bovine collagen is susceptible to host collagenase and can induce immune response. Autologous collagen has become recently available, but it is less effective as a medialization agent. The study examines human skin fibroblasts growing in culture. Human skin bioptates were taken from the retroauricular area. Fibroblasts in culture were tested for scar contractility and ability to produce type I collagen (by flow cytometry with labeled antibodies). After five passages in culture the cells produced normal type I collagen, exhibited normal contractility, and did not induce no tumors in nude mice.
Author List
Berke G, Blumin J, Sebastian J, Keller G, Revazova EAuthor
Joel H. Blumin MD Chief, Professor in the Otolaryngology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AnimalsCattle
Cell Transplantation
Cells, Cultured
Collagen
Fibroblasts
Humans
Mice
Mice, Nude
Safety
Transplantation, Autologous
Transplantation, Heterologous
Vocal Cords
