A naturally occurring, warm-reactive macroglobulin specific for papain-treated human platelets: preliminary characterization. Am J Hematol 1986 Feb;21(2):189-99
Date
02/01/1986Pubmed ID
3942132DOI
10.1002/ajh.2830210208Scopus ID
2-s2.0-0022647295 (requires institutional sign-in at Scopus site) 1 CitationAbstract
Sera from 28 of the 113 normal children and adults (25%) studied were found to contain an immunoglobulin capable of causing complement-dependent lysis of normal platelets treated with small quantities of papain. This factor reacts equally well at 4 degrees C and at 37 degrees C with a determinant induced on platelets from normal subjects by treatment with papain or bromelain, but not by trypsin, chymotrypsin, or neuraminidase. It does not bind to red cells treated with any of these enzymes. The site(s) for which the factor was specific could not be induced on platelets from six patients with type I Glanzmann's thrombasthenia (lacking glycoproteins IIb and IIIa), in contrast to platelets from each of 20 normal donors. Isolation and characterization of the factor has been difficult because of its intolerance to chemical and physical manipulation. In 11 of the 20 individuals studied, however, it was found to have the properties of an IgM immunoglobulin. The factor appears to be different from any previously described, naturally occurring human immunoglobulin. It has not yet been shown to be associated with any disease state, but in the presence of complement, it is capable of causing profound damage to platelets previously subjected to minimal proteolysis, and the possibility that it can provoke platelet destruction in some conditions deserves further study.
Author List
Gill JC, Carlson P, Kunicki TJ, Aster RHMESH terms used to index this publication - Major topics in bold
AdolescentAdult
Aged
Autoantibodies
Blood Platelets
Cell Survival
Child
Child, Preschool
Humans
Immunoglobulin G
Immunoglobulin M
Middle Aged
Papain
Thrombasthenia
