Faculty Collaboration Database

Evaluation of cerebrospinal fluid mononuclear cells obtained from children with acute lymphocytic leukemia: advantages of combining cytomorphology and terminal deoxynucleotidyl transferase. Am J Clin Pathol 1983 Nov;80(5):666-70

Date

11/01/1983

Pubmed ID

6579834

DOI

10.1093/ajcp/80.5.666

Scopus ID

2-s2.0-0021082218 (requires institutional sign-in at Scopus site)   17 Citations

Abstract

Sixty cerebrospinal fluid (CSF) samples were obtained from 28 children with terminal deoxynucleotidyl transferase (TdT) positive acute lymphocytic leukemia (ALL). Cell morphology was evaluated using Wright's stained cytocentrifugation prepared slides. The presence of nuclear TdT was detected by an immunofluorescent (IF) assay. Evaluation of CSF mononuclear cells using these two methods simultaneously allowed us to differentiate between leukemic and nonleukemic pleocytosis. Agreement between cytomorphology and TdT in identifying CNS lymphoblasts was found in 55 of 60 samples. Seventy-two per cent of the TdT-positive samples were obtained from children with CSF cell counts less than 10 WBC/mm3. We recommend that these two methods be used in conjunction when evaluating CSF mononuclear cells from children with TdT positive ALL.

Author List

Casper JT, Lauer SJ, Kirchner PA, Gottschall JL, Camitta BM

MESH terms used to index this publication - Major topics in bold

Child
Child, Preschool
DNA Nucleotidylexotransferase
DNA Nucleotidyltransferases
Humans
Leukemia, Lymphoid
Leukocyte Count
Leukocytes
Lymphocytes
Male
Monocytes