Medical College of Wisconsin
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Evaluation of cerebrospinal fluid mononuclear cells obtained from children with acute lymphocytic leukemia: advantages of combining cytomorphology and terminal deoxynucleotidyl transferase. Am J Clin Pathol 1983 Nov;80(5):666-70 PMID: 6579834

Pubmed ID



Sixty cerebrospinal fluid (CSF) samples were obtained from 28 children with terminal deoxynucleotidyl transferase (TdT) positive acute lymphocytic leukemia (ALL). Cell morphology was evaluated using Wright's stained cytocentrifugation prepared slides. The presence of nuclear TdT was detected by an immunofluorescent (IF) assay. Evaluation of CSF mononuclear cells using these two methods simultaneously allowed us to differentiate between leukemic and nonleukemic pleocytosis. Agreement between cytomorphology and TdT in identifying CNS lymphoblasts was found in 55 of 60 samples. Seventy-two per cent of the TdT-positive samples were obtained from children with CSF cell counts less than 10 WBC/mm3. We recommend that these two methods be used in conjunction when evaluating CSF mononuclear cells from children with TdT positive ALL.

Author List

Casper JT, Lauer SJ, Kirchner PA, Gottschall JL, Camitta BM


Bruce M. Camitta MD Clinical Professor in the Medicine department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Child, Preschool
DNA Nucleotidylexotransferase
DNA Nucleotidyltransferases
Leukemia, Lymphoid
Leukocyte Count
jenkins-FCD Prod-353 9ccd8489072cb19f5b9f808bb23ed672c582f41e