Faculty Collaboration Database

Multicity outbreak of carbapenem-resistant Acinetobacter baumannii isolates producing the carbapenemase OXA-40. Antimicrob Agents Chemother 2006 Sep;50(9):2941-5

Date

08/31/2006

Pubmed ID

16940085

Pubmed Central ID

PMC1563549

DOI

10.1128/AAC.00116-06

Scopus ID

2-s2.0-33748700252 (requires institutional sign-in at Scopus site)   181 Citations

Abstract

During 2005 we detected a multicity outbreak of infections or colonization due to high-level imipenem-resistant Acinetobacter baumannii (MIC, 64 microg/ml). One hundred isolates from diverse sources were obtained from seven acute-care hospitals and two extended-care facilities; 97% of the isolates belonged to one clone. Susceptibility testing of the first 42 isolates (January to April 2005) revealed broad resistance profiles. Half of the isolates were susceptible to ceftazidime, with many isolates susceptible only to colistin. The level of AmpC beta-lactamase expression was stronger in isolates resistant to ceftazidime. PCR and subsequent nucleotide sequencing analysis identified bla(OXA-40). The presence of an OXA-40 beta-lactamase in these isolates correlated with the carbapenem resistance. By Southern blot analysis, a bla(OXA-40)-specific probe revealed that the gene was both plasmid and chromosomally located. This is the first time in the United States that such carbapenem resistance in A. baumannii has been attributable to a carbapenemase.

Author List

Lolans K, Rice TW, Munoz-Price LS, Quinn JP

MESH terms used to index this publication - Major topics in bold

Acinetobacter Infections
Acinetobacter baumannii
Anti-Bacterial Agents
Carbapenems
Ceftazidime
Chicago
Disease Outbreaks
Electrophoresis, Gel, Pulsed-Field
Humans
Indiana
Isoelectric Focusing
Microbial Sensitivity Tests
Polymerase Chain Reaction
beta-Lactam Resistance
beta-Lactamases