Faculty Collaboration Database

Differential effect of pH upon cyclic-ADP-ribose and nicotinate-adenine dinucleotide phosphate-induced Ca2+ release systems. Biochem J 1998 Nov 01;335 ( Pt 3)(Pt 3):499-504

Date

10/31/1998

Pubmed ID

9794787

Pubmed Central ID

PMC1219808

DOI

10.1042/bj3350499

Scopus ID

2-s2.0-0032211756 (requires institutional sign-in at Scopus site)   20 Citations

Abstract

We investigated the pH dependence and the effects of thimerosal and dithiothreitol (DTT) upon the Ca2+ release induced by cADP-ribose (cADPR) and nicotinate-adenine dinucleotide phosphate (NAADP) in sea urchin egg homogenates. Both Ca2+ release triggered by cADPR and the binding of [3H]cADPR to sea urchin egg homogenates were decreased by alkalization of the assay media from pH 7.2 to 8.9. In contrast, NAADP-triggered Ca2+ release was not influenced by changes in pH. The Ca2+ release induced by cADPR was potentiated by thimerosal and inhibited by DTT, but neither thimerosal nor DTT had any effect upon the Ca2+ release induced by NAADP. We conclude that cADPR-sensitive Ca2+-release mechanisms are dependent on pH of the assay media and are sensitive to thiol group modification. On the other hand, these functional properties are not shared by NAADP-regulated Ca2+ channels.

Author List

Chini EN, Liang M, Dousa TP

MESH terms used to index this publication - Major topics in bold

Adenosine Diphosphate Ribose
Animals
Caffeine
Calcium
Cyclic ADP-Ribose
Female
Hydrogen-Ion Concentration
Kinetics
NADP
Oocytes
Sea Urchins
Thimerosal
Tritium