Faculty Collaboration Database

Modulation of vascular tone in renal microcirculation by erythrocytes: role of EDRF. Am J Physiol 1993 Jan;264(1 Pt 2):H190-5

Date

01/01/1993

Pubmed ID

8430846

DOI

10.1152/ajpheart.1993.264.1.H190

Scopus ID

2-s2.0-0027404794 (requires institutional sign-in at Scopus site)   40 Citations

Abstract

The effect of erythrocytes (red blood cells, RBC) on vascular tone in the renal microcirculation was examined using the juxtamedullary nephron microvascular preparation perfused in vitro with a physiological salt solution containing 5% albumin. The basal diameters of the arcuate, interlobular, proximal, and distal afferent arterioles averaged 444 +/- 24, 74 +/- 3, 29 +/- 1, and 19 +/- 1 micron, respectively, when perfused with a cell-free solution at a pressure of 80 mmHg. The diameters of the arcuate and interlobular arteries increased by 14 +/- 4 and 13 +/- 4%, respectively, whereas the diameter of the proximal and distal portions of the afferent arterioles decreased by 7 +/- 2% when perfusion pressure was elevated from 80 to 160 mmHg. The addition of RBC to the perfusate reduced the basal diameters of interlobular and afferent arterioles by 11 +/- 4 and 15 +/- 3%, respectively. The maximal vasoconstrictor response was seen after the addition of only 1% RBC to the perfusate. Removal of platelets did not block the vasoconstrictor response to addition of RBC to the perfusate. The role of endothelium-derived relaxing factor (EDRF) in the vasoconstrictor response to RBC was studied by addition of nitric oxide synthase inhibitor, N omega-nitro-L-arginine (L-NNA, 100 microM) to the perfusate. L-NNA reduced the basal diameters of interlobular and afferent arterioles by 7 +/- 3 and 9 +/- 3%, respectively, and abolished the vasoconstrictor response to RBC. L-NNA had no effect on the pressure-diameter relationships of the preglomerular vasculature when added to perfusates already containing RBC.(ABSTRACT TRUNCATED AT 250 WORDS)

Author List

Imig JD, Gebremedhin D, Harder DR, Roman RJ

MESH terms used to index this publication - Major topics in bold

Animals
Arginine
Blood Platelets
Blood Vessels
Erythrocytes
Male
Microcirculation
Nitric Oxide
Nitroarginine
Oxygen
Oxyhemoglobins
Partial Pressure
Rats
Rats, Sprague-Dawley
Renal Circulation
Vasoconstriction
Vasomotor System