Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Successful formation of a chimeric human thymus allograft following transplantation of cultured postnatal human thymus. J Immunol 1997 Jan 15;158(2):998-1005 PMID: 8993022

Pubmed ID

8993022

Abstract

Transplantation of cultured postnatal human thymus was performed in a patient with complete DiGeorge syndrome. Biopsy of the graft 3 mo after implantation revealed normal CD1+ thymocytes in thymic cortical epithelial regions and CD1- thymocytes in thymic medullary epithelial regions, respectively. HLA analysis of graft thymocyte and thymic microenvironment components demonstrated that developing thymocytes and thymic macrophages were recipient derived, while thymic epithelial components were of donor origin. The patient, who initially had no T cells and had profoundly defective T cell function, developed normal T cell responses to mitogens and Ags, tolerance to donor in a mixed lymphocyte reaction, and normal Ab titers after tetanus toxoid and pneumovax immunization. Thus, transplantation of cultured postnatal human thymic tissue in humans can form functional chimeric thymic tissue, and may provide a strategy to reconstitute the peripheral T cell pool in select congenital and acquired immune deficiency syndromes.

Author List

Markert ML, Kostyu DD, Ward FE, McLaughlin TM, Watson TJ, Buckley RH, Schiff SE, Ungerleider RM, Gaynor JW, Oldham KT, Mahaffey SM, Ballow M, Driscoll DA, Hale LP, Haynes BF

Author

Keith T. Oldham MD Professor in the Surgery department at Medical College of Wisconsin




Scopus

2-s2.0-0031568004   56 Citations

MESH terms used to index this publication - Major topics in bold

Chimera
DiGeorge Syndrome
Graft Survival
Humans
Infant
Organ Culture Techniques
Thymus Gland
Transplantation, Homologous
jenkins-FCD Prod-310 bff9d975ec7f2d302586822146c2801dd4449aad