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Determination of neutrophil antigen gene frequencies in five ethnic groups by polymerase chain reaction with sequence-specific primers. Transfusion 1996 Oct;36(10):895-9

Date

10/01/1996

Pubmed ID

8863777

DOI

10.1046/j.1537-2995.1996.361097017176.x

Scopus ID

2-s2.0-0029849007   79 Citations

Abstract

BACKGROUND: The granulocyte antigens NA1 and NA2 are the two recognized allelic forms of Fc gamma receptor IIIB. These antigens are clinically relevant, because they are the most frequent targets of neutrophil antibodies in alloimmune neonatal neutropenia, transfusion-related acute lung injury, and chronic benign autoimmune neutropenia of infancy.

STUDY DESIGN AND METHODS: A genotyping assay for NA1 and NA2 using polymerase chain reaction with sequence-specific forward and reverse oligonucleotide primers has been developed and validated. Genomic DNA was isolated from the peripheral blood of 478 unrelated individuals of five ethnic groups and used as template for NA genotyping.

RESULTS: A validation study of 22 serologically typed samples (2 NA1/NA1, 10 NA1/NA2, and 10 NA2/NA2) was performed. A concordance rate of 100 percent (22/22 samples) was observed between the genotyping assay and serologic typing. In the genotyping study conducted, the NA1 and NA2 gene frequencies observed were 0.31 and 0.69 for African Americans, 0.30 and 0.70 for Asian Indians, 0.37 and 0.63 for whites, 0.53 and 0.47 for Hispanics, and 0.55 and 0.45 for Native Americans, respectively.

CONCLUSION: Polymerase chain reaction with sequence-specific primers provides a simple and rapid alternative to neutrophil antigen typing by serologic tests. The NA1 and NA2 gene frequencies observed in Asian Indians and African American populations are similar to those observed in white populations, while those observed in Native American and Hispanic populations are more similar to those previously reported for Asian populations.

Author List

Hessner MJ, Curtis BR, Endean DJ, Aster RH

Authors

Richard H. Aster MD Professor in the Medicine department at Medical College of Wisconsin
Brian Curtis PhD Director in the Platelet & Neutrophil Immunology Laboratory department at BloodCenter of Wisconsin
Martin J. Hessner PhD Professor in the Pediatrics department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Ethnic Groups
Gene Frequency
Humans
Immunophenotyping
Isoantigens
Neutrophils
Polymerase Chain Reaction
United States
jenkins-FCD Prod-409 d1e206b0be345926047b0d9c353c78a4cce4058b