Faculty Collaboration Database

Photochemical purging of autologous bone marrow grafts: assessment of damage to stem cells and the microenvironment in long-term marrow cultures. Bone Marrow Transplant 1997 Jul;20(1):27-31

Date

07/01/1997

Pubmed ID

9232252

DOI

10.1038/sj.bmt.1700837

Scopus ID

2-s2.0-0030843823 (requires institutional sign-in at Scopus site)   9 Citations

Abstract

Toxicity of merocyanine 540 (MC540) was assessed in long-term (Dexter-type) bone marrow cultures and in a short-term in vitro clonal assay of fibroblast colony-forming cells (CFU-F). Exposure of freshly explanted mouse bone marrow cells to MC540 (15 micrograms/ml) and white light (fluence: 126 kJ/m2) reduced CFU-F by approximately 2 logs but did not abrogate the cells' capacity to establish and maintain long-term bone marrow cultures. Fat cells were rare or absence in adherent layers established with photosensitized bone marrow cells but the cultures' capacity to generate non-adherent cells, granulocyte/macrophage progenitors (CFU-GM), and early erythroid progenitors (BFU-E) was only moderately (28-36%) reduced.

Author List

Kubo Y, Sieber F

Author

Fritz Sieber PhD Professor in the Pediatrics department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Bone Marrow Purging
Bone Marrow Transplantation
Cells, Cultured
Hematopoietic Stem Cells
Mice
Photosensitizing Agents
Pyrimidinones
Stromal Cells
Transplantation, Autologous