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Impact of postantibiotic effect on bacterial adherence to vascular prostheses. J Surg Res 1990 Apr;48(4):373-8



Pubmed ID




Scopus ID

2-s2.0-0025357942   5 Citations


The brief exposure of bacteria to high antibiotic concentrations can result in prolonged suppression of bacterial growth termed postantibiotic effect (PAE). A pathogenic Staphylococcus epidermidis strain (RP-62) was exposed to 2X and 6X minimum inhibitory concentrations (MIC) of cefazolin, vancomycin, or rifampin for 1 hr and incubated for 2 to 24 hr in inhibitory-free broth. PAE was defined as the difference in time required for a 1.0 log increase in test (T) vs control (C) cultures (PAE = T-C). PAE was observed only for rifampin: 6 hr at 2X MIC and 8 hr at 6X MIC. Bacterial adherence to Dacron grafts was calculated in PAE vs control cultures by a quantitative culture technique for graft specimens incubated 2 to 24 hr. A demonstrable PAE and its impact on adherence were found to be both antimicrobial and concentration dependent. A significant decrease in staphylococcal adherence to velour-knitted Dacron was demonstrated by rifampin at 2X MIC (P less than 0.05) and 6X MIC (P less than 0.01). This phenomena may be useful in reducing bacterial adherence and colonization of bioprosthetics in the perioperative period. Preoperative suppression of staphylococcal skin flora by high dose antimicrobials can alter the capacity of these organisms to adhere to vascular prosthetic grafts and, if incorporated into antibiotic prophylaxis regimens, may reduce graft colonization.

Author List

Schmitt DD, Edmiston CE, Krepel C, Gohr C, Seabrook GR, Bandyk DF, Towne JB


Gary R. Seabrook MD Chief, Professor in the Surgery department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Anti-Bacterial Agents
Bacterial Adhesion
Blood Vessel Prosthesis
Cells, Cultured
Microscopy, Electron, Scanning
Polyethylene Terephthalates
Staphylococcal Infections
Staphylococcus epidermidis
jenkins-FCD Prod-468 69a93cef3257f26b866d455c1d2b2d0f28382f14