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Evaluation of an antimicrobial surgical glove to inactivate live human immunodeficiency virus following simulated glove puncture. Surgery 2013 Feb;153(2):225-33

Date

10/13/2012

Pubmed ID

23059114

DOI

10.1016/j.surg.2012.07.034

Scopus ID

2-s2.0-84872486172   2 Citations

Abstract

BACKGROUND: Percutaneous injuries associated with cutting instruments, needles, and other sharps (eg, metallic meshes, bone fragments, etc) occur commonly during surgical procedures, exposing members of surgical teams to the risk for contamination by blood-borne pathogens. This study evaluated the efficacy of an innovative integrated antimicrobial glove to reduce transmission of the human immunodeficiency virus (HIV) following a simulated surgical-glove puncture injury.

METHODS: A pneumatically activated puncturing apparatus was used in a surgical-glove perforation model to evaluate the passage of live HIV-1 virus transferred via a contaminated blood-laden needle, using a reference (standard double-layer glove) and an antimicrobial benzalkonium chloride (BKC) surgical glove. The study used 2 experimental designs. In method A, 10 replicates were used in 2 cycles to compare the mean viral load following passage through standard and antimicrobial gloves. In method B, 10 replicates were pooled into 3 aliquots and were used to assess viral passage though standard and antimicrobial test gloves. In both methods, viral viability was assessed by observing the cytopathic effects in human lymphocytic C8166 T-cell tissue culture. Concurrent viral and cell culture viability controls were run in parallel with the experiment's studies.

RESULTS: All controls involving tissue culture and viral viability were performed according to study design. Mean HIV viral loads (log(10)TCID(50)) were significantly reduced (P < .01) following passage through the BKC surgical glove compared to passage through the nonantimicrobial glove. The reduction (log reduction and percent viral reduction) of the HIV virus ranged from 1.96 to 2.4 and from 98.9% to 99.6%, respectively, following simulated surgical-glove perforation.

CONCLUSION: Sharps injuries in the operating room pose a significant occupational risk for surgical practitioners. The findings of this study suggest that an innovative antimicrobial glove was effective at significantly (P < .01) reducing the risk for blood-borne virus transfer in a model of simulated glove perforation.

Author List

Edmiston CE Jr, Zhou SS, Hoerner P, Krikorian R, Krepel CJ, Lewis BD, Brown KR, Rossi PJ, Graham MB, Seabrook GR

Authors

Kellie R. Brown MD Professor in the Surgery department at Medical College of Wisconsin
Mary Beth Graham MD Associate Chief, Professor in the Medicine department at Medical College of Wisconsin
Brian D. Lewis MD Professor in the Surgery department at Medical College of Wisconsin
Peter J. Rossi MD Chief, Associate Professor in the Surgery department at Medical College of Wisconsin
Gary R. Seabrook MD Chief, Professor in the Surgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Anti-Infective Agents
Benzalkonium Compounds
Gloves, Surgical
HIV
HIV Infections
Humans
In Vitro Techniques
Microbial Viability
Needles
Needlestick Injuries
Occupational Injuries
Viral Load
Virus Inactivation
jenkins-FCD Prod-398 336d56a365602aa89dcc112f077233607d6a5abc