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Evidence for respiratory neuromodulator interdependence after cholinergic disruption in the ventral respiratory column. Respir Physiol Neurobiol 2015 Jan 01;205:7-15 PMID: 25262584 PMCID: PMC4341919


Reverse dialysis of the muscarinic receptor antagonist, atropine (ATR, 50 mM), into the pre-Bötzinger Complex region of the ventral respiratory column (VRC) of awake and sleeping goats increases breathing frequency and serotonin (5-HT), substance P (SP), glycine, and GABA concentrations in the effluent dialysate. Herein, we report data from goats in which we reverse dialyzed 5 mM ATR or specific antagonists of M2 or M3 muscarinic receptors into the VRC. The effects on frequency of all three antagonists were not significantly different from time control studies. 5 mM ATR and the M3 antagonist increased SP sevenfold less than 50 mM ATR. The antagonists had no effect on 5-HT, glycine, and/or GABA, suggesting that the increases in glycine and GABA with 50 mM ATR were secondary to the larger increases in 5-HT and/or SP. These data are suggestive of neuromodulator interdependence, whereby attenuation of one neuromodulator is compensated for by local changes in other neuromodulators to stabilize breathing.

Author List

Muere C, Neumueller S, Miller J, Olesiak S, Hodges MR, Pan L, Forster HV


Matthew R. Hodges PhD Associate Professor in the Physiology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Chromatography, High Pressure Liquid
Muscarinic Antagonists
Neurotransmitter Agents
Receptors, Muscarinic
Respiratory Center
Respiratory Physiological Phenomena

View this publication's entry at the Pubmed website PMID: 25262584
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