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Excitatory amino acids and rod photoreceptor disc shedding: analysis using specific agonists. Exp Eye Res 1988 Oct;47(4):609-20 PMID: 3053226

Pubmed ID

3053226

Abstract

L-Glutamate and L-aspartate stimulate photoreceptor disc shedding. In order to evaluate the possible involvement of a receptor, we examined the effects of specific excitatory amino acid agonists on rod photoreceptor disc shedding and neural retinal toxicity. Using eyecups from both Xenopus laevis and Rana pipiens, we found that kainate, quisqualate, and N-methyl-D-aspartate (NMDA) were all neurotoxic, but that kainate caused a more extensive inner retinal lesion. Kainate also caused disc shedding at concentrations as low as 10 microM; dihydrokainate, a structural analogue, was at least 100-fold less potent. In contrast, quisqualate induced disc shedding only at concentrations above 5.0 mM, and NMDA had no effect on disc shedding at any concentration examined. Our results suggest that excitatory amino acids act via a receptor of the kainate type to effect disc shedding. The mechanism in the retina or photoreceptor-pigment epithelial complex by which an excitatory amino acid receptor system influences disc shedding remains to be identified.

Author List

Besharse JC, Spratt G

Author

Joseph C. Besharse PhD Director, Professor in the Ophthalmology and Visual Sciences department at Medical College of Wisconsin




Scopus

2-s2.0-0023726593   15 Citations

MESH terms used to index this publication - Major topics in bold

Amino Acids
Animals
Aspartic Acid
Dose-Response Relationship, Drug
In Vitro Techniques
Kainic Acid
Light
N-Methylaspartate
Oxadiazoles
Phagosomes
Photoreceptor Cells
Pigment Epithelium of Eye
Quisqualic Acid
Rana pipiens
Xenopus laevis
jenkins-FCD Prod-353 9ccd8489072cb19f5b9f808bb23ed672c582f41e