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Effects on breathing of agonists to μ-opioid or GABA receptors dialyzed into the ventral respiratory column of awake and sleeping goats. Respir Physiol Neurobiol 2017 05;239:10-25

Date

02/01/2017

Pubmed ID

28137700

Pubmed Central ID

PMC5996971

DOI

10.1016/j.resp.2017.01.007

Scopus ID

2-s2.0-85011649848   2 Citations

Abstract

Pulmonary ventilation (V̇) in awake and sleeping goats does not change when antagonists to several excitatory G protein-coupled receptors are dialyzed unilaterally into the ventral respiratory column (VRC). Concomitant changes in excitatory neuromodulators in the effluent mock cerebral spinal fluid (mCSF) suggest neuromodulatory compensation. Herein, we studied neuromodulatory compensation during dialysis of agonists to inhibitory G protein-coupled or ionotropic receptors into the VRC. Microtubules were implanted into the VRC of goats for dialysis of mCSF mixed with agonists to either μ-opioid (DAMGO) or GABA (muscimol) receptors. We found: (1) V̇ decreased during unilateral but increased during bilateral dialysis of DAMGO, (2) dialyses of DAMGO destabilized breathing, (3) unilateral dialysis of muscimol increased V̇ and (4) dialysis of DAMGO decreased GABA in the effluent mCSF. We conclude: (1) neuromodulatory compensation can occur during altered inhibitory neuromodulator receptor activity, and (2) the mechanism of compensation differs between G protein-coupled excitatory and inhibitory receptors and between G protein-coupled and inotropic inhibitory receptors.

Author List

Langer TM 3rd, Neumueller SE, Crumley E, Burgraff NJ, Talwar S, Hodges MR, Pan L, Forster HV

Authors

Hubert V. Forster PhD Professor in the Physiology department at Medical College of Wisconsin
Matthew R. Hodges PhD Associate Professor in the Physiology department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Analgesics, Opioid
Animals
Dialysis
Dose-Response Relationship, Drug
Enkephalin, Ala(2)-MePhe(4)-Gly(5)-
Female
Functional Laterality
GABA-A Receptor Agonists
Goats
Muscimol
Neurotransmitter Agents
Respiration
Respiratory Center
Sleep
Wakefulness
jenkins-FCD Prod-399 190a069c593fb5498b7fcd942f44b7bc9cdc7ea1