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Single and combinatorial chromatin coupling events underlies the function of transcript factor Krüppel-like factor 11 in the regulation of gene networks. BMC Mol Biol 2014 May 25;15:10 PMID: 24885560 PMCID: PMC4049485

Pubmed ID

24885560

DOI

10.1186/1471-2199-15-10

Abstract

BACKGROUND: Krüppel-like factors (KLFs) are a group of master regulators of gene expression conserved from flies to human. However, scant information is available on either the mechanisms or functional impact of the coupling of KLF proteins to chromatin remodeling machines, a deterministic step in transcriptional regulation.

RESULTS AND DISCUSSION: In the current study, we use genome-wide analyses of chromatin immunoprecipitation (ChIP-on-Chip) and Affymetrix-based expression profiling to gain insight into how KLF11, a human transcription factor involved in tumor suppression and metabolic diseases, works by coupling to three co-factor groups: the Sin3-histone deacetylase system, WD40-domain containing proteins, and the HP1-histone methyltransferase system. Our results reveal that KLF11 regulates distinct gene networks involved in metabolism and growth by using single or combinatorial coupling events.

CONCLUSION: This study, the first of its type for any KLF protein, reveals that interactions with multiple chromatin systems are required for the full gene regulatory function of these proteins.

Author List

Calvo E, Grzenda A, Lomberk G, Mathison A, Iovanna J, Urrutia R

Authors

Gwen Lomberk PhD Associate Professor in the Surgery department at Medical College of Wisconsin
Angela Mathison PhD Assistant Professor in the Surgery department at Medical College of Wisconsin
Raul A. Urrutia MD Center Director, Professor in the Surgery department at Medical College of Wisconsin




Scopus

2-s2.0-84902078010   2 Citations

MESH terms used to index this publication - Major topics in bold

Cell Cycle Proteins
Cells, Cultured
Chromatin
Chromatin Assembly and Disassembly
Gene Expression Regulation
Gene Regulatory Networks
Genome-Wide Association Study
Histone-Lysine N-Methyltransferase
Humans
Repressor Proteins
Transcription Factors
Transcription, Genetic
jenkins-FCD Prod-334 7d79545a27550c5c05320b79c4a9173528a20b0a