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Haploid embryonic stem cell lines derived from androgenetic and parthenogenetic rat blastocysts. J Reprod Dev 2017 Dec 15;63(6):611-616 PMID: 28824040 PMCID: PMC5735273

Pubmed ID

28824040

Abstract

The present study was conducted to establish haploid embryonic stem (ES) cell lines using fluorescent marker-carrying rats. In the first series, 7 ES cell lines were established from 26 androgenetic haploid blastocysts. However, only 1 ES cell line (ahES-2) was found to contain haploid cells (1n = 20 + X) by fluorescence-activated cell sorting (FACS) and karyotypic analyses. No chimeras were detected among the 10 fetuses and 41 offspring derived from blastocyst injection with the FACS-purified haploid cells. In the second series, 2 ES cell lines containing haploid cells (13% in phES-1 and 1% in phES-2) were established from 2 parthenogenetic haploid blastocysts. Only the phES-2 cell population was purified by repeated FACS to obtain 33% haploid cells. Following blastocyst injection with the FACS-purified haploid cells, no chimera was observed among the 11 fetuses; however, 1 chimeric male was found among the 47 offspring. Although haploid rat ES cell lines can be established from both blastocyst sources, FACS purification may be necessary for maintenance and chimera production.

Author List

Hirabayashi M, Hara H, Goto T, Takizawa A, Dwinell MR, Yamanaka T, Hochi S, Nakauchi H

Author

Melinda R. Dwinell PhD Center Associate Director, Associate Professor in the Physiology department at Medical College of Wisconsin




Scopus

2-s2.0-85040115487

MESH terms used to index this publication - Major topics in bold

Animals
Blastocyst
Cell Line
Embryonic Stem Cells
Parthenogenesis
Rats
jenkins-FCD Prod-299 9ef562391eceb2b8f95265c767fbba1ce5a52fd6