Faculty Collaboration Database

Polarized expression of members of the solute carrier SLC19A gene family of water-soluble multivitamin transporters: implications for physiological function. Biochem J 2003 Nov 15;376(Pt 1):43-8

Date

11/07/2003

Pubmed ID

14602044

Pubmed Central ID

PMC1223768

DOI

10.1042/BJ20031220

Scopus ID

2-s2.0-0344825276 (requires institutional sign-in at Scopus site)   38 Citations

Abstract

Humans lack biochemical pathways for the synthesis of the micro-nutrients thiamine and folate. Cellular requirements are met through membrane transport activity, which is mediated by proteins of the SLC19A gene family. By using live-cell confocal imaging methods to resolve the localization of all SLC19A family members, we show that the two human thiamine transporters are differentially targeted in polarized cells, establishing a vectorial transport system. Such polarization decreases functional redundancy between transporter isoforms and allows for independent regulation of thiamine import and export pathways in cells.

Author List

Boulware MJ, Subramanian VS, Said HM, Marchant JS

Author

Jonathan S. Marchant PhD Chair, Professor in the Cell Biology, Neurobiology and Anatomy department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Biological Transport
Cell Line
Cell Polarity
Dogs
Folic Acid
Humans
Membrane Transport Proteins
Microscopy, Confocal
Solubility
Thiamine
Water