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Characterization of Stress and Innate Immunity Resistance of Wild-Type and Δ. Infect Immun 2018 02;86(2) PMID: 29158430 PMCID: PMC5778354

Pubmed ID

29158430

Abstract

is a causative agent of Lyme disease, the most common arthropod-borne disease in the United States. evades host immune defenses to establish a persistent, disseminated infection. Previous work showed that P66-deficient (Δ) is cleared quickly after inoculation in mice. We demonstrate that the Δ strain is rapidly cleared from the skin inoculation site prior to dissemination. The rapid clearance of Δ bacteria is not due to inherent defects in multiple properties that might affect infectivity: bacterial outer membrane integrity, motility, chemotactic response, or nutrient acquisition. This led us to the hypothesis that P66 has a role in mouse cathelicidin-related antimicrobial peptide (mCRAMP; a major skin antimicrobial peptide) and/or neutrophil evasion. Neither wild-type (WT) nor Δ was susceptible to mCRAMP. To examine the role of neutrophil evasion, we administered neutrophil-depleting antibody anti-Ly6G (1A8) to C3H/HeN mice and subsequently monitored the course of infection. Δ mutants were unable to establish infection in neutrophil-depleted mice, suggesting that the important role of P66 during early infection is through another mechanism. Neutrophil depletion did not affect WT bacterial burdens in the skin (inoculation site), ear, heart, or tibiotarsal joint at early time points postinoculation. This was unexpected given that prior studies demonstrated neutrophils phagocytose and kill These data, together with our previous work, suggest that despite the ability of host innate defenses to kill , individual innate immune mechanisms have limited contributions to controlling early infection in the laboratory model used.

Author List

Curtis MW, Hahn BL, Zhang K, Li C, Robinson RT, Coburn J

Author

Jenifer Coburn PhD Professor in the Medicine department at Medical College of Wisconsin




Scopus

2-s2.0-85040966556

MESH terms used to index this publication - Major topics in bold

Animals
Bacterial Proteins
Borrelia burgdorferi
Disease Models, Animal
Female
Gene Deletion
Immunity, Innate
Lyme Disease
Mice, Inbred C3H
Neutrophils
Porins
jenkins-FCD Prod-296 4db9d02597e0a2e889e230f853b641c12f1c3ee3