Identification of cell surface markers to differentiate rat endothelial and fibroblast cells using lectin arrays and LC-ESI-MS/MS. Anal Chem 2008 Nov 01;80(21):8269-75
Date
09/30/2008Pubmed ID
18821777Pubmed Central ID
PMC2950091DOI
10.1021/ac801390bScopus ID
2-s2.0-55549110908 (requires institutional sign-in at Scopus site) 8 CitationsAbstract
Vascular endothelial cells located at the inner surface of blood vessels are a key component in angiogenesis and are employed as a primary cell type in the study of angiogenesis. These endothelial cells are, however, easily contaminated with fibroblast cells, which are located in proximity to the endothelial cells, during their isolation from tissue. It is thus important to find markers to distinguish the two cell types. In the present work, lectin arrays were prepared using aldehyde-terminated self-assembled monolayers (SAMs) and utilized to explore cell surface carbohydrate expression patterns on endothelial and fibroblast cells. It was found that the lectins Griffonia simplicifolia II (GS II) and Ulex europaeus agglutinin I (UEA I) selectively bind to rat fibroblast cells and not to rat endothelial cells. GS II-binding glycoproteins on fibroblast cells, which are potential cell surface markers to differentiate endothelial and fibroblast cells, were captured on a GS II lectin column and analyzed by LC-ESI-MS/MS. Six candidate cell surface glycoproteins were identified. Differential expression was confirmed by Western blot analysis for two of these proteins, lysosome-associated membrane glycoprotein-1 and transmembrane glycoprotein NMB.
Author List
Lee JE, Mirza SP, Didier DN, Scalf M, Olivier M, Greene AS, Smith LMMESH terms used to index this publication - Major topics in bold
AnimalsBiomarkers
Carbohydrates
Cell Membrane
Cells, Cultured
Chromatography, Liquid
Endothelial Cells
Fibroblasts
Lectins
Protein Array Analysis
Rats
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
