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Soluble proteins and haptens on bone marrow-derived dendritic cells are presented to host CD4 T cells in an MHC-restricted manner. Int Immunol 2002 May;14(5):493-502



Pubmed ID




Scopus ID

2-s2.0-0036094973 (requires institutional sign-in at Scopus site)   4 Citations


Because of their potent antigen-presenting capacity, dendritic cells (DC) have been used extensively in immunotherapy protocols. Our purpose was to functionally characterize mouse bone marrow-derived DC (BMDC) in vitro (in protein antigen- and hapten-specific assays) and in vivo (injecting soluble protein- and hapten-pulsed DC) to determine their suitability for the generation of T(h) cell responses. Furthermore, we determined whether there is cross-presentation on MHC class II molecules during in vivo protein and hapten sensitization. Co-culture of protein-pulsed [with hen egg lysozyme (HEL) or with pigeon cytochrome c (CYT)] DC with T cells from HEL- or CYT- sensitized mice induced antigen-specific T cell proliferation, but compared to cultured Langerhans cells (LC), BMDC required higher protein antigen-pulsing concentrations (100 microg and 1 mg/ml). In contrast, at low protein concentrations (10 microg/ml), BMDC stimulated an HEL-specific hybridoma very efficiently. Using an in vitro T cell proliferation assay and in vivo delayed-type hypersensitivity and contact sensitivity assays, we found that protein- and hapten-pulsed BMDC were able to sensitize syngeneic but not allogeneic hosts. Furthermore, if we injected BALB/c- and C57BL/6-derived HEL-pulsed BMDC into F1 mice, specific secondary proliferation of primed T cells occurred only when antigen-pulsed stimulator cells syngeneic to the injected BMDC were used. Using this model system we found that soluble proteins and haptens are presented by injected BMDC to host T cells in an MHC-restricted manner in vivo.

Author List

Olasz EB, Linton J, Katz SI


Edit Olasz MD, PhD Associate Professor in the Dermatology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Antigen Presentation
Bone Marrow Cells
Cytochrome c Group
Dendritic Cells
Egg Proteins
Histocompatibility Antigens
Hypersensitivity, Delayed
Langerhans Cells
Membrane Glycoproteins
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred Strains
Picryl Chloride
T-Lymphocyte Subsets
T-Lymphocytes, Helper-Inducer