Alteration of secretory leukocyte protease inhibitor in human myometrium during labor. Am J Obstet Gynecol 2009 Mar;200(3):311.e1-311.e10
Date
03/04/2009Pubmed ID
19254589DOI
10.1016/j.ajog.2008.10.045Scopus ID
2-s2.0-60849117794 (requires institutional sign-in at Scopus site) 3 CitationsAbstract
OBJECTIVE: Secretory leukocyte protease inhibitor (SLPI) has been shown to have antimicrobial and antiinflammatory properties. The aim of this study was to verify its expression in human myometrium.
STUDY DESIGN: Myometrium was obtained at time of cesarean delivery with (n = 9) or without (n = 11) labor. Expression of SLPI was detected using real-time polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry. SLPI expression relative to nuclear factor-kappaB p65 subunit was compared between subjects. SLPI response to inflammatory mediators was studied in myometrial explants.
RESULTS: SLPI was predominantly localized in the nuclei of myocytes and colocalized with CD68(+) macrophages. The nuclear immunoreactivity of SLPI was increased after the onset of labor and was associated with increased nuclear translocation of nuclear factor-kappaB p65 subunit. Treatment with lipopolysaccharide, interleukin-1beta, or tumor necrosis factor-alpha increased SLPI messenger RNA and protein concentrations slightly in myometrium explants.
CONCLUSION: SLPI was expressed in human myometrium and increased after the onset of labor.
Author List
Li A, Lee RH, Felix JC, Minoo P, Goodwin TMAuthor
Juan Felix MD Vice Chair, Director, Professor in the Pathology department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdolescentAdult
Antigens, CD
Antigens, Differentiation, Myelomonocytic
Cells, Cultured
Decidua
Extraembryonic Membranes
Female
Humans
Immunohistochemistry
Infant, Newborn
Interleukin-1beta
Labor, Obstetric
Lipopolysaccharides
Macrophages
Male
Myometrium
Pregnancy
RNA, Messenger
Reverse Transcriptase Polymerase Chain Reaction
Secretory Leukocyte Peptidase Inhibitor
Transcription Factor RelA
Tumor Necrosis Factor-alpha
Young Adult
