Faculty Collaboration Database

Expression of cyclin protein after thermal skin injury in a guinea pig model. J Burn Care Rehabil 1997;18(4):292-8

Date

07/01/1997

Pubmed ID

9261693

DOI

10.1097/00004630-199707000-00003

Scopus ID

2-s2.0-0030806862 (requires institutional sign-in at Scopus site)   4 Citations

Abstract

In this study, an immunohistochemical stain for cyclin was used to quantitate proliferating elements in hair follicles at the edge of and within thermal burn areas. Biopsy specimens from thermal injury in the guinea pig (day 1 through day 28) were sectioned and stained with MIB-1 antibody, which recognizes cyclin, a protein expressed during epithelial cell proliferation. At the edge of the burn, 89 +/- 6.1 (SD) cells per medium power field (x 10, mpf) were MIB-1-positive on days 1 through 16. On day 17, the number of positive cells increased, reaching peak values on days 20 to 28 (271 +/- 12.7 cells/mpf). Within the burn, minimal staining was observed from day 1 to day 15 (12.7 +/- 1.6 cells/mpf). Thereafter the number of MIB-1-positive cells increased and plateaued with an average of 96.4 +/- 9.0 cells/mpf on days 20 through 28. In conclusion, immunohistochemical staining of dermal biopsy specimens with MIB-1 antibody may provide a quantitative method for the evaluation of tissue damage and healing after thermal injury.

Author List

DeCherney AH, Dougherty WR, Felix JC, Rodgers KE, Girgis W, Abiko M, diZerega GS

Author

Juan Felix MD Vice Chair, Director, Professor in the Pathology department at Medical College of Wisconsin

MESH terms used to index this publication - Major topics in bold

Animals
Antibodies, Monoclonal
Burns
Cell Division
Cyclins
Disease Models, Animal
Guinea Pigs
Hair Follicle
Immunohistochemistry
Male
Skin