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The G(0)/G(1) switch gene 2 regulates adipose lipolysis through association with adipose triglyceride lipase. Cell Metab 2010 Mar 03;11(3):194-205

Date

03/04/2010

Pubmed ID

20197052

Pubmed Central ID

PMC3658843

DOI

10.1016/j.cmet.2010.02.003

Scopus ID

2-s2.0-77249118270   312 Citations

Abstract

Adipose triglyceride lipase (ATGL) is the rate-limiting enzyme for triacylglycerol (TAG) hydrolysis in adipocytes. The precise mechanisms whereby ATGL is regulated remain uncertain. Here, we demonstrate that a protein encoded by G(0)/G(1) switch gene 2 (G0S2) is a selective regulator of ATGL. G0S2 is highly expressed in adipose tissue and differentiated adipocytes. When overexpressed in HeLa cells, G0S2 localizes to lipid droplets and prevents their degradation mediated by ATGL. Moreover, G0S2 specifically interacts with ATGL through the hydrophobic domain of G0S2 and the patatin-like domain of ATGL. More importantly, interaction with G0S2 inhibits ATGL TAG hydrolase activity. Knockdown of endogenous G0S2 accelerates basal and stimulated lipolysis in adipocytes, whereas overexpression of G0S2 diminishes the rate of lipolysis in both adipocytes and adipose tissue explants. Thus, G0S2 functions to attenuate ATGL action both in vitro and in vivo and by this mechanism regulates TAG hydrolysis.

Author List

Yang X, Lu X, Lomb├Ęs M, Rha GB, Chi YI, Guerin TM, Smart EJ, Liu J

Author

Young-In Chi PhD Assistant Professor in the Surgery department at Medical College of Wisconsin




MESH terms used to index this publication - Major topics in bold

Adipocytes
Adipose Tissue
Animals
Carboxylic Ester Hydrolases
Cell Cycle
Cell Cycle Proteins
Gene Expression Regulation
HeLa Cells
Humans
Lipase
Lipids
Lipolysis
Mice
Protein Interaction Domains and Motifs
Vacuoles