Processing of normal antibody-coated sheep erythrocytes by mouse peritoneal macrophages. Immunology 1972 Sep;23(3):361-74
Date
09/01/1972Pubmed ID
4679835Pubmed Central ID
PMC1407938Scopus ID
2-s2.0-0015394807 (requires institutional sign-in at Scopus site) 2 CitationsAbstract
The processing by mouse peritoneal macrophages of normal sheep red blood cells (SRBC) or SRBC coated with 19S or 7S antibody was investigated by measuring (a) total uptake of SRBC, (b) changes in subcellular distribution of antigen during in vitro culture, and (c) immunogenicity of SRBC-containing macrophages. Most of the ingested antigen was found to be bound to lysosomal membranes. Its concentration decreased rapidly during the first 5 hours of culture. Although SRBC uptake was increased by the addition of either class of antibody, 7S antibody suppressed and 19S antibody enhanced the immunogenicity of SRBC-containing macrophages. Rate of intracellular antigen degradation and production of low-density antigen did not correlate with macrophage immunogenicity. While the enhancing effect of 19S antibody probably was due to an increase in the percentage of SRBC-ingesting cells, suppression by 7S antibody may have been related to the creation of a distinct class of heavily laden macrophages with reduced immunogenic efficiency.
Author List
Sohnle PG, Sussdorf DHMESH terms used to index this publication - Major topics in bold
AnimalsAntibodies
Antibody Formation
Antibody Specificity
Antigens
Cell Fractionation
Cells, Cultured
Centrifugation, Density Gradient
Erythrocytes
Immune Sera
Injections, Intraperitoneal
Macrophages
Membranes
Mice
Peritoneum
Phagocytosis
Protein Binding
Sheep
Thioglycolates
