Medical College of Wisconsin
CTSICores SearchResearch InformaticsREDCap

Crystallization of HMG-CoA reductase from Pseudomonas mevalonii. Acta Crystallogr D Biol Crystallogr 1995 May 01;51(Pt 3):386-9



Pubmed ID




Scopus ID

2-s2.0-0029140384 (requires institutional sign-in at Scopus site)   10 Citations


Crystals of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase from Pseudomonas mevalonii have been grown by vapor diffusion in hanging drops at pH 6.7 using ammonium sulfate as the precipitant. Serial dilution seeding and manipulation of glycerol concentration were both used to obtain crystals larger than 1.0 mm. The crystals are cubic, space group I4(1)32, with a = 229.4 A. A V(m) value of 2.71 A(3) Da(-l) indicates 96 molecules per unit cell with two molecules in the asymmetric unit. These crystals diffract to 2.8 A with conventional X-ray sources, and beyond 2.4 A with synchrotron radiation.

Author List

Lawrence CM, Chi YI, Rodwell VW, Stauffacher CV


Young-In Chi PhD Assistant Professor in the Surgery department at Medical College of Wisconsin