Crystallization and preliminary X-ray diffraction analysis of staphylococcal enterotoxin type C. Proteins 1992 Apr;13(2):152-7
Date
04/01/1992Pubmed ID
1620697DOI
10.1002/prot.340130208Scopus ID
2-s2.0-0026691079 (requires institutional sign-in at Scopus site) 11 CitationsAbstract
The Type C staphylococcal enterotoxin produced by Staphylococcus aureus strain FRI-909 has been crystallized using a combination of two precipitants, ammonium sulfate and polyethylene glycol 400, with the addition of small amounts of detergent. Two related crystal forms have been obtained, one triclinic, and one tetragonal, both with one toxin molecule per asymmetric unit. These crystals are stable for at least 75 hr in the X-ray beam and diffract to at least 2.2 and 2.6 A, respectively. The triclinic crystals have unit cell parameters a = 38.5 A, b = 43.7 A, c = 36.9 A, and interaxial angles alpha = 99.9 degrees, beta = 95.8 degrees, and gamma = 98.5 degrees. The tetragonal crystals are of space group P4(1)22 with unit cell parameters a = 43.4 A and c = 278.0 A.
Author List
Bohach GA, Chi YI, Stauffacher CVAuthor
Young-In Chi PhD Assistant Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
CrystallizationEnterotoxins
Molecular Structure
Protein Conformation
Structure-Activity Relationship
X-Ray Diffraction
