Binding site identification and structure determination of protein-ligand complexes by NMR a semiautomated approach. Methods Enzymol 2011;493:241-75
Date
03/05/2011Pubmed ID
21371594Pubmed Central ID
PMC3635485DOI
10.1016/B978-0-12-381274-2.00010-8Scopus ID
2-s2.0-79952366609 (requires institutional sign-in at Scopus site) 62 CitationsAbstract
Over the last 15 years, the role of NMR spectroscopy in the lead identification and optimization stages of pharmaceutical drug discovery has steadily increased. NMR occupies a unique niche in the biophysical analysis of drug-like compounds because of its ability to identify binding sites, affinities, and ligand poses at the level of individual amino acids without necessarily solving the structure of the protein-ligand complex. However, it can also provide structures of flexible proteins and low-affinity (K(d)>10(-6)M) complexes, which often fail to crystallize. This chapter emphasizes a throughput-focused protocol that aims to identify practical aspects of binding site characterization, automated and semiautomated NMR assignment methods, and structure determination of protein-ligand complexes by NMR.
Author List
Ziarek JJ, Peterson FC, Lytle BL, Volkman BFAuthors
Francis C. Peterson PhD Professor in the Biochemistry department at Medical College of WisconsinBrian F. Volkman PhD Professor in the Biochemistry department at Medical College of Wisconsin
MESH terms used to index this publication - Major topics in bold
Automation, LaboratoryBinding Sites
Drug Design
Drug Discovery
Hydrogen Bonding
Ligands
Magnetic Resonance Spectroscopy
Models, Molecular
Nuclear Magnetic Resonance, Biomolecular
Proteins
Software