Tumor load in patients with follicular lymphoma post stem cell transplantation may correlate with clinical course. Bone Marrow Transplant 2003 Aug;32(3):287-91
Date
07/15/2003Pubmed ID
12858200DOI
10.1038/sj.bmt.1704130Scopus ID
2-s2.0-0043171049 (requires institutional sign-in at Scopus site) 14 CitationsAbstract
The purpose of this study was to evaluate if the tumor load, as determined by a real-time quantitative PCR (RQ-PCR) assay, correlated with the clinical course of follicular lymphoma patients after stem cell transplantation (SCT). Cryopreserved bone marrow and/or peripheral blood samples obtained at different time intervals after SCT from 11 patients (seven allogeneic, T-cell depleted/four autologous) were tested for tumor load, as defined by t(14;18) positive cells/total cells, using RQ-PCR. None of the six patients who remained in remission had samples with a tumor load >0.01% after SCT, although fluctuating tumor loads of </=0.01% were observed in three of these patients. In contrast, four of the five patients (three allogeneic/two autologous) with relapsed/progressive disease had increasing tumor loads of >0.01% after SCT (0/6 vs 4/5, P<0.02, Fisher's exact). Our results suggest that RQ-PCR measurable tumor load >0.01% after SCT may correlate with relapsed/progressive disease. Prospective studies with greater numbers of cases are indicated to better determine the critical tumor load that predicts poor outcome after SCT with RQ-PCR.
Author List
Chang CC, Bredeson C, Juckett M, Logan B, Keever-Taylor CAAuthor
Brent R. Logan PhD Director, Professor in the Institute for Health and Equity department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
AdultDisease Progression
Female
Hematopoietic Stem Cell Transplantation
Humans
Lymphoma, Follicular
Male
Middle Aged
Molecular Diagnostic Techniques
Neoplasm, Residual
Polymerase Chain Reaction
Retrospective Studies
Time Factors
Translocation, Genetic
