Further characterization of delta aroA delta virG Shigella flexneri 2a strain CVD 1203 as a mucosal Shigella vaccine and as a live-vector vaccine for delivering antigens of enterotoxigenic Escherichia coli. Infect Immun 1996 Jan;64(1):23-7
Date
01/01/1996Pubmed ID
8557344Pubmed Central ID
PMC173722DOI
10.1128/iai.64.1.23-27.1996Scopus ID
2-s2.0-0029671022 (requires institutional sign-in at Scopus site) 70 CitationsAbstract
The use of attenuated delta aroA delta virG Shigella flexneri 2a strain CVD 1203 as a live vector for enterotoxigenic Escherichia coli (ETEC) antigens is reported. CVD 1203 alone or expressing colonization factor antigen fimbriae and CS3 fibrillae of ETEC was given to guinea pigs and mice, orogastrically (o.g.) or intranasally (i.n.). CVD 1203 given i.n. elicited high titers of antilipopolysaccharide (anti-LPS) immunoglobulin A (IgA) and was protective in guinea pigs against a homologous conjunctival challenge. Whereas a strong IgA response against colonization factor antigen CS3, and Shigella LPS was detected in tears and serum of guinea pigs after o.g. or i.n. immunization, the i.n. route elicited significantly higher antibody titers. A strong serum IgG response was also observed against the ETEC antigens, although no serum anti-LPS IgG response was detected. The immune response in mice followed a pattern similar to that in guinea pigs, and the difference between the responses after o.g. and i.n. administration was even more remarkable.
Author List
Noriega FR, Losonsky G, Wang JY, Formal SB, Levine MMAuthor
Paul Joseph Pearson MD, PhD Chief, Professor in the Surgery department at Medical College of WisconsinMESH terms used to index this publication - Major topics in bold
3-Phosphoshikimate 1-CarboxyvinyltransferaseAlkyl and Aryl Transferases
Animals
Antigens, Bacterial
Bacterial Proteins
Bacterial Vaccines
DNA-Binding Proteins
Drug Administration Routes
Dysentery, Bacillary
Escherichia coli
Escherichia coli Infections
Fimbriae Proteins
Gastric Mucosa
Guinea Pigs
Immunoglobulin A
Immunoglobulin G
Immunologic Tests
Mice
Mouth Mucosa
Nasal Mucosa
Sequence Deletion
Shigella flexneri
Transcription Factors
Transferases
Vaccines, Attenuated
Vaccines, Synthetic
